SAPRA Biomedicals

Committed to Provide Excellent Tools for Biomedical Research

Welcome to Sapra Biomedicals. Our main goal is to always achieve a high level of customer satisfaction with the services and products that we provide. This simple approach has effectively fueled our growth since we opened our doors in 2023. Please browse our site to discover what we’re all about.

We Offer The Following Products and Services

We will provide CRISPR knockout mutants of human and mouse cell lines

Construction of genetically defined knockout mutants of mammalian cell lines

We are capable of deleting the genes of your interest in any of the mammalian cell lines.
CRISPR/Cas9 approach will be used in knocking out the genes
The mutants of following cell lines can be constructed and provided to you within a matter of six weeks.
- Human embryonic kidney cell line 293 (HEK293)
- Human heart/aorta smooth muscle fibroblast T/G HA-VSMC
The mutants of any of the other mammalian cell lines can also be constructed and provided to you within a matter of eight weeks.
You name the gene, we will construct the mutant, confirm the accuracy of deletion, and send to you the mutant.
The oligo primers, PCR conditions, antibodies, and immunoblotting conditions will be shared with you so that you will be capable of independently verifying the mutations.

We will construct defined, knockout mutants of pathogenic bacterial agents

Construction of genetically defined mutants and complemented mutants of bacterial agents

We are capable of deleting the genes of your interest of the following bacterial agents:
- Actinobacilus pleuropneumoniae
- Francisella tularensis LVS
- Escherichia coli
- Histophilus somni
- Neisseria gonorrhoeae
You name the gene, we will construct the mutant, confirm the accuracy of deletion, and send to you the mutant and the complemented mutant.
The oligo primers and PCR conditions will be shared with you so that you will be capable of independently verifying the mutations.
The mutants and complemented mutants can be provided within 3 to 4 weeks after obtaining your order.

Genetic manipulation of bacterial agents to overexpress heterologous or homologous proteins

We are capable of overexpressing the genes of your interest of the following bacterial agents:
- Actinobacilus pleuropneumoniae
- Francisella tularensis LVS
- Histophilus somni
- Neisseria gonorrhoeae
You name the gene, we will modify the bacterium to overexpress your gene, and send to you the genetically manipulated strain.
The manipulated strains can be provided within 3 to 4 weeks after obtaining your order.

Construction of genetically defined mutants of fungal agents

We are capable of deleting the genes of your interest of the following fungal agents:
- Candida albicans
- Candida auris
- Candida glabrata
- Candida lusitaniae
You name the gene, we will construct the mutant, confirm the accuracy of deletion, and send to you the mutant.
The oligo primers and PCR conditions will be shared with you so that you will be capable of independently verifying the mutations.
The mutants can be provided within 4 to 5 weeks after obtaining your order.

We will test the safety and efficacy of drug candidates against specific pathogenic bacterial agents

Testing the efficacy of your newly discovered drug candidates in mammalian cell lines

We are capable of testing the cytotoxicity of any of your drug candidates in mammalian cell lines, including the following:
- Human embryonic kidney cell line 293 (HEK293)
- Human heart/aorta smooth muscle fibroblast T/G HA-VSMC
We are capable of testing the efficacy of your drug candidates in restoration of functional deficits of the following mitochondrial mutants:
- HEK293 with disrupted complex I of the electron transport chain
- HEK293 with disrupted complex II of the electron transport chain